Product Details：

Product description：

MULTI^TM Nuclease is a non-restrictive recombinant endonuclease derived from Serratia Marcescens. It can degrade all forms of DNA and RNA (double-stranded, single-stranded, linear, circular and supercoiled) under a very wide range of conditions (6 M Urea, 0.1 M Guanidine HCl, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF), and generate 3-5 oligonucleotide residue fragments containing 5'-phosphate ends, which are widely used to remove nucleic acids in biological products.

MULTI^TM Nuclease is expressed and purified in Escherichia coli through genetic engineering. No antibiotics are added during the production process, and it is of no animal origin. It meets GMP standards and minimizes the risk of contamination. At the same time, MULTI^TM Nuclease has the characteristics of high purity, strong activity, and no protease activity. It can be widely used in scientific research, cell therapy, viral vector vaccines and other fields.

Figure 1. Structural view of the MULTI^TM Nuclease

Unit defnition

One unit of MULTI^TM Nuclease is defned as the amount of enzyme that causes a change in absorbance at 260 nm of 1.0 absorption units within 30 minutes. One unit of MULTI^TM Nuclease also corresponds approximately to the amount of enzyme required to completely digest 37 μg of DNA in 30 minutes under standard assay conditions

Storage

Store it under sterile conditions at -20℃ to -80℃ upon receiving for at least 12 months.

Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles

Standard MULTITM Nuclease Activity Assay

Application of MULTI^TM Nuclease

1、Removal of host nucleic acid during vaccine production

2、Removal of host nucleic acid during the purification of AAV, lentivirus and oncolytic virus

3、Reduce the viscosity of the lysate and improve the efficiency of protein purification

4、Used for the preparation of electrophoresis and chromatographic samples to improve resolution

5、Prevent aggregation of peripheral blood mononuclear cells (PBMC) in cell therapy or vaccine research

Application examples of MULTI^TM Nuclease

Figure 2. MULTITM Nuclease can efficiently degrade DNA

Figure 3. Comparison between MULTI^TM Nuclease and competitor in adenovirus purification

Figure 4. Nuclease residues can be efficiently removed by one-step chromatography