T7 RNA polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase , which can specifically recognize the double stranded sequence of T7 promoter (5'-TAATACGACTCACTATA-3'), and synthesize RNA in the 5'→3' direction with single or double stranded DNA downstream of T7 promoter as template and NTP as raw material.
Product Details
Product Details：
Product name | Lot Number | Product size |
T7 RNA polymerase | N1C0101 | 1000 U |
Product description
T7 RNA polymerase is a DNA-dependent RNA polymerase , which can specifically recognize the double stranded sequence of T7 promoter (5'-TAATACGACTCACTATA-3'), and synthesize RNA in the 5'3' direction with single or double stranded DNA downstream of T7 promoter as template and NTP as raw material.
This product is a recombinant protein purified from E.coli, and the purification process meets GMP standards. It can specifically recognize T7 promoters and use PCR products or linear plasmids as templates to efficiently catalyze mRNA, siRNA, sgRNA, etc. It can be widely used in scientific research, biomedicine and other fields.
Figure 1. Structural view of the T7 RNA polymerase
Protein Characterization
Source | T7 phage |
Host | E. coli |
Molecular weight | 99 kDa |
Isoelectric point（pI） | 6.8 |
Purity | ≥95.0%（SDS-PAGE） |
Sterility | not detectable |
Endotoxin | < 0.05EU/1000U |
Storage | -20℃ |
Unit defnition
Under the conditions of 37°C and pH 8.0, the amount of enzyme used to incorporate 1 nmol of AMP into oligonucleotides within 1 h is defined as one unit of activity (U).
Application examples of MULTITM Nuclease
A: RNA synthesized by this product, B: The synthesized RNA was successfully used for crystal structure analysis.
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Guangzhou XinBaiTai Biotechnology Co., Ltd.
E-mail：qiuchenlin@doubllebio.com
Address：No. 9, Ruifa Road, Science City, Huangpu District, Guangzhou
Tel：020-32053717
Zip code：510700
Guangzhou xinbaitai Biotechnology Co., Ltd. 粤ICP备2021157220号-1
